Journal: PLoS ONE

Article Title: Recombinant Dengue 2 Virus NS3 Helicase Protein Enhances Antibody and T-Cell Response of Purified Inactivated Vaccine

doi: 10.1371/journal.pone.0152811

Figure Lengend Snippet: Groups of vaccinated mice were tested for IFN-γ responses using a standard ELISPOT assay. Mouse spleen cells collected on Day 35 after first immunization, were stimulated with overlapping peptide pools spanning the entire M, E and NS3 protein. Responses are presented as number of spots per one million cells. Results indicate the mean of 3 mice with error bars equal to one standard deviation. PIV = purified inactivated vaccine, Pro = protease, Hel = helicase, E = envelope, M = membrane.

Article Snippet: The spots were counted using the AID ELISPOT Reader (AID Autoimmun Diagnostika, Germany).

Techniques: Enzyme-linked Immunospot, Standard Deviation, Purification, Membrane

Journal: PLoS ONE

Article Title: Recombinant Dengue 2 Virus NS3 Helicase Protein Enhances Antibody and T-Cell Response of Purified Inactivated Vaccine

doi: 10.1371/journal.pone.0152811

Figure Lengend Snippet: Whole spleen cells (a) or spleen cells depleted with either CD4 or CD8 subset (b, c), were stained with anti-mouse CD4 or CD8 monoclonal antibodies. The % of each subset is shown. IFN-γ response from whole or subset- depleted spleen cells was measured by ELISPOT assay (d). Results represent number of spots per million cells. Control = media without peptides.

Article Snippet: The spots were counted using the AID ELISPOT Reader (AID Autoimmun Diagnostika, Germany).

Techniques: Staining, Bioprocessing, Enzyme-linked Immunospot, Control

Journal: Viruses

Article Title: Co-Deletion of A238L and EP402R Genes from a Genotype IX African Swine Fever Virus Results in Partial Attenuation and Protection in Swine

doi: 10.3390/v14092024

Figure Lengend Snippet: ASFV-specific antibody and cellular immune responses: percentage of blocking by p72-specific serum antibodies after mock-immunisation with PBS ( A ) or immunisation with ASFV-Ke-∆EP402R∆A238L ( B ) determined using a commercial blocking ELISA. Mean+/−SEM of percentage blocking in PBS- or ASFV-Ke-∆EP402R∆A238L-immunised groups ( C ). IFNγ ELISPOT using PBMC isolated from animals at day 28 after immunisation with PBS (blue) or ASFV-Ke-∆EP402R∆A238L (red) and stimulation with either medium or ASFV-Ke wild type (wt) infection at an MOI of 0.1 ( D ).

Article Snippet: The spots were counted using the AID classic ELISPOT reader (AID AutoImmun Diagnostika GmbH.

Techniques: Blocking Assay, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Isolation, Infection